Anti-ErbB2/HER2 Protein A Scaffold, Agarose-Immobilized (CAT#: AFB-25LY)

Datasheet

DESCRIPTION	CATALOG #	SIZE	PRICE
Anti-Human ErbB2/HER2 Protein A scaffold, Agarose-Immobilized	AFB-25LY	1mg	Please Inquiry

PRODUCT INFORMATION

Product Overview
The Anti-ErbB2/HER2 Protein A Scaffold molecule is a highly specific affinity ligand selected against the extra cellular domain of HER2. The molecule is immobilized on agarose at the unique C-terminal cysteine. The Agarose-Immobilized Anti-HER2 Protein A Scaffold molecule is excellent for immunoprecipitation studies of HER2 in cell extracts or other solutions that contain HER2 proteins. In addition, the Anti-HER2 Protein A Scaffold molecule may also be used for affinity chromatography.

Specific Activity
Anti-HER2 Protein A scaffold molecule binds to extracellular domain of human epidermal growth factor receptor 2 (HER2). Cross reactivity with other species has not been tested.

Storage
At +4°C. Avoid freezing. There is no decrease in performance of the Agarose-Immobilized Protein A Scaffold molecule after storage for 2 weeks at +37°C.

Antigen Description
The human epidermal growth factor receptor HER2 (Her2/neu, ErbB2, or c-erb-b2) is a growth factor receptor that is expressed on many cell types. HER2-proteins are overexpressed in a number of cancer types and assemble in homo- or heterodimers to form functional signaling units. When overexpressed, homo- or heterodimers of HER2 cause increased proliferation, decreased apoptosis, enhanced tumor cell motility and neo-angiogenesis-all being traits strongly associated with tumor formation. Thus, the her2 gene is considered an oncogene.

Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Synonyms
NEU; NGL; HER2; TKR1; CD340; HER-2; HER-2/neu; EC 2.7.10.1; C-erbB-2; erbB-2; c-erb B2/neu protein; erbB-2; herstatin; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); NEU proto-oncogene; MLN 19; CD340 antigen; ERBB2

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